TM7SF2: ENSGT00390000000417.1.Euteleostomitransmembrane 7 superfamily member 20.00000086378213117571Euteleostomi110.223082300.01567963782137898Actinopterygii1100.942103380.000000002395283183671489341Osteoglossocephalai1210.210937100.01817863782131489341Osteoglossocephalai1300.13942650.999826378213186625Clupeocephala1400.14541600.2990463782131489388Euteleosteomorpha1500.2207061000.11792637821341705Protacanthopterygii160ENSHHUP000000816750.150236637821362062Hucho_huchoENSHHUG00000047479tm7sf2_ENSHHUENSHHUP0000008167517ENSELUP000000368230.25729963782138010Esox_luciusENSELUG00000001224ENSELUP00000036823180.318270630.03029363782131489872Percomorphaceae1900.07657900.9999363782131489872Percomorphaceae11000.01919300.9999463782131489872Percomorphaceae11100.01046000.9994463782131489872Percomorphaceae11200.05076110.9998763782131489908Ovalentaria11300.071250580.9994563782131489913Atherinomorphae11400.056673810.99998637821328738Cyprinodontiformes11500.171039930.9998763782138087Cyprinodontoidei11600.003578990.6453463782138087Cyprinodontoidei1170ENSFHEP000000243600.14773763782138078Fundulus_heteroclitusENSFHEG00000005841tm7sf2_FUNHEENSFHEP00000024360118ENSCVAP000000155360.198228637821328743Cyprinodon_variegatusENSCVAG00000018398tm7sf2_CYPVAENSCVAP000000155361190.233511970.999716378213586240Poeciliinae12000.0296981000.9999763782138082Xiphophorus1210ENSXMAP000000299000.00258263782138083Xiphophorus_maculatusENSXMAG00000026147tm7sf2_XIPMAENSXMAP00000029900122ENSXCOP000000075570.022218637821332473Xiphophorus_couchianusENSXCOG00000005824tm7sf2_ENSXCOENSXCOP000000075571230.0527301000.9997963782138080Poecilia12400.033587370.9994363782138080Poecilia12500.009572370.9611663782138080Poecilia1260ENSPFOP000000190840.000000637821348698Poecilia_formosaENSPFOG00000018968tm7sf2_POEFOENSPFOP00000019084127ENSPLAP000000176180.024870637821348699Poecilia_latipinnaENSPLAG00000022085tm7sf2_ENSPLAENSPLAP00000017618128ENSPMEP000000004880.010240637821348701Poecilia_mexicanaENSPMEG00000001283tm7sf2_ENSPMEENSPMEP00000000488129ENSPREP000000079900.03946963782138081Poecilia_reticulataENSPREG00000005429tm7sf2_POEREENSPREP00000007990130ENSKMAP000000154560.282727637821337003Kryptolebias_marmoratusENSKMAG00000011538tm7sf2_KRYMAENSKMAP000000154561310.4232691000.09129963782138089Oryzias13200.0517671000.9999363782138090Oryzias_latipes13300.001654100163782138090Oryzias_latipes1340ENSORLP000200189560.00251163782138090Oryzias_latipesENSORLG00020019922tm7sf2_ORYLAENSORLP00020018956135ENSORLP000150019050.01457263782138090Oryzias_latipesENSORLG00015002554tm7sf2_ORYLAENSORLP00015001905136ENSORLP000000134100.03447563782138090Oryzias_latipesENSORLG00000010698tm7sf2_ORYLAENSORLP00000013410137ENSOMEP000000263910.118075637821330732Oryzias_melastigmaENSOMEG00000008189tm7sf2_ORYMEENSOMEP00000026391138ENSGWIP000000147310.5287976378213441366Gouania_willdenowiENSGWIG00000008104tm7sf2_ENSGWIENSGWIP000000147311390.300913991637821331031Tetraodontidae1400ENSTRUP000000029290.127349637821331033Takifugu_rubripesENSTRUG00000001262tm7sf2_TAKRUENSTRUP00000002929141ENSTNIP000000137190.178797637821399883Tetraodon_nigroviridisENSTNIG00000010795tm7sf2_TETNGENSTNIP00000013719142ENSCSEP000000241150.6181646378213244447Cynoglossus_semilaevisENSCSEG00000015386tm7sf2_CYNSEENSCSEP000000241151430.01304200.9998263782131489872Percomorphaceae14400.00000000.9999363782131489872Percomorphaceae14500.00277810.9994463782131489872Percomorphaceae14600.079735690.99948637821350370Anabantoidei1470ENSATEP000000116090.153996637821364144Anabas_testudineusENSATEG00000008123tm7sf2_ANATEENSATEP00000011609148ENSBSLP000000236460.2426776378213158456Betta_splendensENSBSLG00000011994tm7sf2_BETSPENSBSLP000000236461490.270406960.99996378213318546Pseudocrenilabrinae15000.030591950.999816378213318546Pseudocrenilabrinae15100.002380320.999686378213319058Haplochromini15200.000000250.999816378213319058Haplochromini15300.007408810.999346378213319058Haplochromini1540ENSMZEP000050179110.0024496378213106582Maylandia_zebraENSMZEG00005013458tm7sf2_ENSMZEENSMZEP00005017911155ENSACLP000000142260.00497963782138154Astatotilapia_callipteraENSACLG00000009702tm7sf2_ASTCAENSACLP00000014226156ENSHBUP000000341690.00489963782138153Haplochromis_burtoniENSHBUG00000021541tm7sf2_HAPBUENSHBUP00000034169157ENSPNYP000000307020.0073566378213303518Pundamilia_nyerereiENSPNYG00000023138tm7sf2_ENSPNYENSPNYP00000030702158ENSNBRP000000163100.022361637821332507Neolamprologus_brichardiENSNBRG00000012593tm7sf2_NEOBRENSNBRP00000016310159ENSONIP000000021980.02258563782138128Oreochromis_niloticusENSONIG00000001754tm7sf2_ORENIENSONIP00000002198160ENSMMOP000000085410.308572637821394237Mola_molaENSMMOG00000006604tm7sf2_MOLMLENSMMOP000000085411610.01167100.9996363782131489872Percomorphaceae16200.00971100.9996163782131489872Percomorphaceae16300.03513830.9999163782131489909Ovalentaria_incertae_sedis16400.134499940.9993637821330863Pomacentridae16500.033711990.99991637821330863Pomacentridae16600.0436641000.9999637821380969Amphiprion1670ENSAOCP000000176780.007308637821380972Amphiprion_ocellarisENSAOCG00000022806tm7sf2_AMPOCENSAOCP00000017678168ENSAPEP000000132600.0125626378213161767Amphiprion_perculaENSAPEG00000009415tm7sf2_AMPPEENSAPEP00000013260169ENSAPOP000000029430.032429637821380966Acanthochromis_polyacanthusENSAPOG00000004405tm7sf2_ENSAPOENSAPOP00000002943170ENSSPAP000000312470.2102576378213144197Stegastes_partitusENSSPAG00000023425tm7sf2_ENSSPAENSSPAP00000031247171ENSPRNP000000521530.2187496378213210632Parambassis_rangaENSPRNG00000024658tm7sf2_ENSPRNENSPRNP000000521531720.03146770.1237863782131489904Carangaria17300.025105100.9999463782131489904Carangaria1740ENSSLDP000000238400.10320563782131841481Seriola_lalandi_dorsalisENSSLDG00000018581tm7sf2_ENSSLDENSSLDP00000023840175ENSSMAP000000320990.189857637821352904Scophthalmus_maximusENSSMAG00000019655tm7sf2_SCOMXENSSMAP00000032099176ENSLCAP000100442270.12009563782138187Lates_calcariferENSLCAG00010020563tm7sf2_LATCAENSLCAP000100442271770.0144200163782131489872Percomorphaceae17800.02214140.9992963782131489922Eupercaria17900.039640750.8845763782138111Perciformes1800ENSCGOP000000116340.104288637821356716Cottoperca_gobioENSCGOG00000005702tm7sf2_COTGOENSCGOP00000011634181ENSGACP000000267990.242997637821369293Gasterosteus_aculeatusENSGACG00000020280tm7sf2_GASACENSGACP000000267991820.03566266163782131489922Eupercaria1830ENSLBEP000000035670.215231637821356723Labrus_bergyltaENSLBEG00000002743tm7sf2_ENSLBEENSLBEP00000003567184ENSLCRP000050547270.2022236378213215358Larimichthys_croceaENSLCRG00005020429tm7sf2_LARCRENSLCRP000050547271850.054352920.99995637821343697Synbranchiformes1860ENSMAMP000000125590.1734046378213205130Mastacembelus_armatusENSMAMG00000008485tm7sf2_ENSMAMENSMAMP00000012559187ENSMALP000000211370.224163637821343700Monopterus_albusENSMALG00000014769tm7sf2_MONALENSMALP00000021137188ENSPMGP000000125270.9260956378213409849Periophthalmus_magnuspinnatusENSPMGG00000010333tm7sf2_ENSPMGENSPMGP000000125271890.20596300.631966378213186634Otomorpha19000.17591800.00602946378213186626Otophysi19100.414857710.590246378213186628Characiphysae1920ENSPNAP000000211490.536497637821342514Pygocentrus_nattereriENSPNAG00000028182tm7sf2_PYGNAENSPNAP00000021149193ENSIPUP000000258701.03396763782137998Ictalurus_punctatusENSIPUG00000017507tm7sf2_ICTPUENSIPUP00000025870194ENSDARP000000421610.52486263782137955Danio_rerioENSDARG00000032816tm7sf2_DANREENSDARP000000421611950.219907980.086761637821332446Clupeiformes1960ENSDCDP000000424390.4631886378213299321Denticeps_clupeoidesENSDCDG00000027273tm7sf2_ENSDCDENSDCDP00000042439197ENSCHAP000000551180.49811163782137950Clupea_harengusENSCHAG00000024979tm7sf2_CLUHAENSCHAP000000551181980.2873171000.0079139637821341712Osteoglossiformes1990ENSSFOP000150307830.4435956378213113540Scleropages_formosusENSSFOG00015019738tm7sf2_SCLFOENSSFOP000150307831100ENSPKIP000000069420.41141663782131676925Paramormyrops_kingsleyaeENSPKIG00000023028tm7sf2_ENSPKIENSPKIP000000069421101ENSGMOP000000202580.86363663782138049Gadus_morhuaENSGMOG00000018857tm7sf2_GADMOENSGMOP000000202581102ENSECRP000000093100.969058637821327687Erpetoichthys_calabaricusENSECRG00000006237tm7sf2_ERPCAENSECRP0000000931011030.22308280.015679637821332523Tetrapoda110400.371334910.10173637821332524Amniota110500.24968820.38087637821332561Sauria110600.3422741000.3639763782138464Cryptodira110700.0855371000.9994363782138486Testudinoidea110800.0242931000.999863782138487Testudinidae11090ENSGAGP000000364670.025085637821338772Gopherus_agassiziiENSGAGG00000025865TM7SF2_ENSGAGENSGAGP000000364671110ENSCABP000000039440.0365696378213106734Chelonoidis_abingdoniiENSCABG00000002977TM7SF2_CHEABENSCABP000000039441111ENSCPBP000000338560.05009463782138478Chrysemys_picta_belliiENSCPBG00000023641TM7SF2_CHRPIENSCPBP000000338561112ENSPSIP000000065390.099946637821313735Pelodiscus_sinensisENSPSIG00000006053TM7SF2_PELSIENSPSIP0000000653911130.372805190.3254163782131329912Episquamata11140ENSSMRP000000136450.458978637821396440Salvator_merianaeENSSMRG00000010607TM7SF2_ENSSMRENSSMRP000000136451115ENSPVIP000000140330.5157386378213103695Pogona_vitticepsENSPVIG00000010844TM7SF2_ENSPVIENSPVIP0000001403311160.741183910.00066219637821332525Theria111700.52791300.1895163782139347Eutheria111800.04777900.4502863782139347Eutheria111900.03847400.9997663782131437010Boreoeutheria112000.02498600.999986378213314146Euarchontoglires112100.008057100.8495363782139443Primates112200.0572631000.999856378213376911Strepsirrhini112300.0545447616378213376915Lemuriformes112400.006605760.807176378213376915Lemuriformes11250ENSPSMP000000010550.03589563782131328070Prolemur_simusENSPSMG00000000737TM7SF2_PROSSENSPSMP000000010551126ENSPCOP000000254330.0531296378213379532Propithecus_coquereliENSPCOG00000024943TM7SF2_PROCOENSPCOP000000254331127ENSMICP000000349040.073231637821330608Microcebus_murinusENSMICG00000016571TM7SF2_MICMUENSMICP000000349041128ENSOGAP000000134340.140397637821330611Otolemur_garnettiiENSOGAG00000015003TM7SF2_OTOGAENSOGAP0000001343411290.115263880.999676378213314293Simiiformes113000.0404254163782139526Catarrhini113100.015544460.9999463782139527Cercopithecidae113200.015063890.9992663782139528Cercopithecinae113300.008545210.9999463782139528Cercopithecinae113400.00245856163782139528Cercopithecinae11350ENSPANP000000005030.00489363782139555Papio_anubisENSPANG00000020755TM7SF2_PAPANENSPANP000000005031136ENSTGEP000000121500.00488663782139565Theropithecus_geladaENSTGEG00000009909TM7SF2_THEGEENSTGEP0000001215011370.00245300.9999363782139528Cercopithecinae113800.00000000.9999163782139528Cercopithecinae113900.009813830.9999563782139539Macaca114000.000000840.9999363782139539Macaca11410ENSMNEP000000268170.00000063782139545Macaca_nemestrinaENSMNEG00000036835TM7SF2_MACNEENSMNEP000000268171142ENSMMUP000000247070.00000063782139544Macaca_mulattaENSMMUG00000018773TM7SF2_MACMUENSMMUP000000247071143ENSMFAP000000388920.00000063782139541Macaca_fascicularisENSMFAG00000038773TM7SF2_MACFAENSMFAP000000388921144ENSMLEP000000305600.00488563782139568Mandrillus_leucophaeusENSMLEG00000039473TM7SF2_MANLEENSMLEP000000305601145ENSCATP000000001690.00000063782139531Cercocebus_atysENSCATG00000000514TM7SF2_CERATENSCATP000000001691146ENSCSAP000000038870.013656637821360711Chlorocebus_sabaeusENSCSAG00000007642TM7SF2_CHLSBENSCSAP0000000388711470.0185731000.999736378213542827Rhinopithecus11480ENSRROP000000294910.000000637821361622Rhinopithecus_roxellanaENSRROG00000038543TM7SF2_RHIROENSRROP000000294911149ENSRBIP000000065010.002450637821361621Rhinopithecus_bietiENSRBIG00000026647TM7SF2_RHIBEENSRBIP0000000650111500.019199320.999936378213207598Homininae115100.007411280.999946378213207598Homininae11520ENSPTRP000000820400.00815163782139598Pan_troglodytesENSPTRG00000003864TM7SF2_PANTRENSPTRP000000820401153ENSP000002792630.01173663782139606Homo_sapiensENSG00000149809TM7SF2_HUMANENSP000002792631154ENSGGOP000000026710.01181963782139595Gorilla_gorilla_gorillaENSGGOG00000002708TM7SF2_GORGOENSGGOP0000000267111550.050728600.9993263782139479Platyrrhini115600.000000150.9998763782139498Cebidae115700.010045430.9999463782139498Cebidae11580ENSSBOP000000082800.023079637821339432Saimiri_boliviensis_boliviensisENSSBOG00000021045TM7SF2_SAIBBENSSBOP000000082801159ENSCCAP000000319340.04283463782131737458Cebus_capucinus_imitatorENSCCAG00000033991TM7SF2_ENSCCAENSCCAP000000319341160ENSCJAP000000044730.03670663782139483Callithrix_jacchusENSCJAG00000002441TM7SF2_CALJAENSCJAP000000044731161ENSANAP000000399240.023118637821337293Aotus_nancymaaeENSANAG00000037332TM7SF2_AOTNAENSANAP0000003992411620.03746300.999926378213314147Glires116300.03974110.7831763782139989Rodentia116400.0291894163782139989Rodentia116500.148566800.9996637821333550Hystricomorpha116600.050376650.99924637821333550Hystricomorpha116700.009682850.36122637821333550Hystricomorpha11680ENSCLAP000000236270.099465637821334839Chinchilla_lanigeraENSCLAG00000016205TM7SF2_CHILAENSCLAP000000236271169ENSODEP000000246870.139532637821310160Octodon_degusENSODEG00000018073TM7SF2_OCTDEENSODEP000000246871170ENSCPOP000000315910.183773637821310141Cavia_porcellusENSCPOG00000014705TM7SF2_CAVPOENSCPOP0000003159111710.0705761000.00095779637821310167Bathyergidae117200.1213491000.99959637821310181Heterocephalus_glaber11730ENSHGLP001000196080.000000637821310181Heterocephalus_glaberENSHGLG00100014455TM7SF2_HETGAENSHGLP001000196081174ENSHGLP000000159310.000000637821310181Heterocephalus_glaberENSHGLG00000011381TM7SF2_HETGAENSHGLP000000159311175ENSFDAP000000165560.1249746378213885580Fukomys_damarensisENSFDAG00000005147TM7SF2_FUKDAENSFDAP000000165561176ENSDORP000000171680.251216637821310020Dipodomys_ordiiENSDORG00000003619Tm7sf2_DIPORENSDORP0000001716811770.059178780.9999163782131963758Myomorpha117800.055673830.390066378213337687Muroidea117900.112828970.999966378213337687Muroidea118000.035835610.999516378213337677Cricetidae118100.008270630.999226378213337677Cricetidae118200.0392841000.85894637821310026Cricetinae118300.0533161000.99998637821310029Cricetulus_griseus118400.0000001000.99991637821310029Cricetulus_griseus11850ENSCGRP000010119520.000000637821310029Cricetulus_griseusENSCGRG00001013460Tm7sf2_CRIGRENSCGRP000010119521186ENSCGRP000150185350.000000637821310029Cricetulus_griseusENSCGRG00015014237Tm7sf2_CRIGRENSCGRP000150185351187ENSCGRP000000024470.000000637821310029Cricetulus_griseusENSCGRG00000001788Tm7sf2_CRIGRENSCGRP000000024471188ENSMAUP000000151330.065052637821310036Mesocricetus_auratusENSMAUG00000014671Tm7sf2_MESAUENSMAUP000000151331189ENSPEMP000000121510.1281956378213230844Peromyscus_maniculatus_bairdiiENSPEMG00000012570Tm7sf2_PERMBENSPEMP000000121511190ENSMOCP000000067150.131533637821379684Microtus_ochrogasterENSMOCG00000006388Tm7sf2_MICOHENSMOCP0000000671511910.039977970.56952637821310066Muridae119200.050997970.99991637821339107Murinae119300.0317591000.99954637821310088Mus119400.0271811000.546336378213862507Mus119500.018655970.999776378213862507Mus119600.002451970.505786378213862507Mus11970MGP_SPRETEiJ_P00496770.004833637821310096Mus_spretusMGP_SPRETEiJ_G0023499Tm7sf2_MUSSPMGP_SPRETEiJ_P00496771198ENSMSIP000000363420.002418637821310103Mus_spicilegusENSMSIG00000030251Tm7sf2_MUSSIENSMSIP000000363421199ENSMUSP000000257130.009636637821310090Mus_musculusENSMUSG00000024799Tm7sf2_MOUSEENSMUSP000000257131200MGP_CAROLIEiJ_P00477170.020626637821310089Mus_caroliMGP_CAROLIEiJ_G0022588Tm7sf2_MUSCRMGP_CAROLIEiJ_P00477171201MGP_PahariEiJ_P00207230.042831637821310093Mus_pahariMGP_PahariEiJ_G0014082Tm7sf2_MUSPAMGP_PahariEiJ_P00207231202ENSRNOP000000284850.067666637821310116Rattus_norvegicusENSRNOG00000020989Tm7sf2_RATENSRNOP000000284851203ENSMUGP000000243360.104414637821310047Meriones_unguiculatusENSMUGG00000020305Tm7sf2_MERUNENSMUGP000000243361204ENSNGAP000000208190.17884363782131026970Nannospalax_galiliENSNGAG00000020187Tm7sf2_NANGAENSNGAP000000208191205ENSJJAP000000214240.196625637821351337Jaculus_jaculusENSJJAG00000021790Tm7sf2_JACJAENSJJAP0000002142412060.18144800.999816378213337730Marmotini120700.00251400.622576378213337730Marmotini12080ENSMMMP000000108440.01485963782139994Marmota_marmota_marmotaENSMMMG00000009623TM7SF2_MARMAENSMMMP000000108441209ENSSTOP000000029660.016991637821343179Ictidomys_tridecemlineatusENSSTOG00000003307TM7SF2_ICTTRENSSTOP000000029661210ENSUPAP000100126420.00740163782139999Urocitellus_parryiiENSUPAG00010010219TM7SF2_UROPRENSUPAP0001001264212110.03928520.999516378213314145Laurasiatheria121200.00000080.999716378213314145Laurasiatheria121300.072784540.98346637821391561Artiodactyla121400.0745111000.9994563782139895Bovidae121500.0193241000.9994963782139963Caprinae12160ENSCHIP000000048510.00993363782139925Capra_hircusENSCHIG00000008956TM7SF2_CAPHIENSCHIP000000048511217ENSOARP000000134830.01267963782139940Ovis_ariesENSOARG00000012581TM7SF2_SHEEPENSOARP0000001348312180.033438770.99983637821327592Bovinae121900.00000022163782139903Bos12200ENSBMUP000000042750.000000637821372004Bos_mutusENSBMUG00000003360TM7SF2_ENSBMUENSBMUP000000042751221ENSBTAP000000622300.00492063782139913Bos_taurusENSBTAG00000005069TM7SF2_BOVINENSBTAP000000622301222ENSBBBP000000243780.009824637821343346Bison_bison_bisonENSBBBG00000005044TM7SF2_ENSBBBENSBBBP000000243781223ENSTTRP000000163460.06750463782139739Tursiops_truncatusENSTTRG00000017244TM7SF2_TURTRENSTTRP000000163461224ENSMLUP000000139960.213330637821359463Myotis_lucifugusENSMLUG00000015357TM7SF2_MYOLUENSMLUP0000001399612250.021759130.999466378213314145Laurasiatheria122600.056050600.99953637821333554Carnivora122700.026263570.999876378213379584Caniformia122800.043188300.999876378213379584Caniformia122900.03258000.9999163782139632Ursidae12300ENSUAMP000000153200.00628563782139643Ursus_americanusENSUAMG00000012270TM7SF2_URSAMENSUAMP000000153201231ENSAMEP000000186810.02871763782139646Ailuropoda_melanoleucaENSAMEG00000017679TM7SF2_AILMEENSAMEP000000186811232ENSMPUP000000121540.07972263782139669Mustela_putorius_furoENSMPUG00000012251TM7SF2_MUSPFENSMPUP0000001215412330.1304821000.9997963782139612Canis_lupus12340ENSCAFP000200187920.0000006378213286419Canis_lupus_dingoENSCAFG00020014933TM7SF2_DINGOENSCAFP000200187921235ENSCAFP000000205570.00000063782139615Canis_lupus_familiarisENSCAFG00000013951TM7SF2_CANLFENSCAFP000000205571236ENSFCAP000000058100.12814763782139685Felis_catusENSFCAG00000006254TM7SF2_FELCAENSFCAP0000000581012370.1497741000.6086263782139789Equus12380ENSECAP000000189930.00231463782139796Equus_caballusENSECAG00000021411TM7SF2_HORSEENSECAP000000189931239ENSEASP000050266990.009945637821383772Equus_asinus_asinusENSEASG00005018217TM7SF2_ENSEASENSEASP000050266991240ENSDNOP000000252510.20888163782139361Dasypus_novemcinctusENSDNOG00000046178TM7SF2_DASNOENSDNOP0000002525112410.0645261000.999936378213311790Afrotheria12420ENSLAFP000000014070.11629363782139785Loxodonta_africanaENSLAFG00000001685TM7SF2_LOXAFENSLAFP000000014071243ENSPCAP000000141950.18015763782139813Procavia_capensisENSPCAG00000015203TM7SF2_PROCAENSPCAP0000001419512440.4622061000.02493363782139263Metatheria124500.221252560.0021345637821338609Diprotodontia12460ENSVURP000100031450.109615637821329139Vombatus_ursinusENSVURG00010002497TM7SF2_VOMURENSVURP000100031451247ENSMEUP000000085830.11695563782139315Macropus_eugeniiENSMEUG00000009398TM7SF2_MACEUENSMEUP000000085831248ENSMODP000000126570.205862637821313616Monodelphis_domesticaENSMODG00000010108TM7SF2_MONDOENSMODP000000126571249ENSXETP000000436431.22875663782138364Xenopus_tropicalisENSXETG00000021121rp9_XENTRENSXETP000000436431250